EyeTech Prep
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Assisting in Surgical Procedures

Aseptic technique, instrumentation, and assisting for minor and intraocular procedures.

Sterile Processing: Cleaning, Sterilization, Monitoring, and the TASS Connection

The assistant learns to keep a field sterile. The technician is expected to explain why each step works, to know which monitor proves what, and to recognize what happens to a patient when reprocessing fails. Ophthalmic instruments are a special case, and the reason is anatomic: the anterior chamber has no lymphatics and no blood supply to flush away a chemical insult. A residue that would be harmless on a skin retractor can destroy corneal endothelium when it is carried into an eye. That is why intraocular instrument reprocessing has its own literature, its own guidelines, and its own named complication.


The sequence is fixed, and no step substitutes for another. Point-of-use care, transport, cleaning, rinsing, inspection, packaging, sterilization, monitoring, then storage. The governing principle is that sterilization cannot penetrate bioburden — steam kills organisms it can reach, and a film of dried blood, cortex, or viscoelastic is a shelter. A perfect cycle run on a dirty instrument produces a sterile-looking instrument that is neither clean nor safe.


Point of use. Decontamination begins in the operating room, not in the back room. Gross soil is wiped away and instruments are kept moist — with a product intended for the purpose, not with saline, whose chloride pits stainless steel. Viscoelastic is the specific enemy: left to dry, sodium hyaluronate becomes a varnish that ordinary washing will not lift. Cannulated items — phacoemulsification and irrigation-aspiration handpieces, cannulas, tubing-fed tips — are flushed immediately with sterile distilled water, and ophthalmic guidance calls for generous volumes, on the order of at least 120 mL through each handpiece and each lumen. Delicate tips are separated from heavy instruments in transport rather than tossed into a common pan.

In the full COT guide

  1. Sterile Processing: Cleaning, Sterilization, Monitoring, and the TASS Connection
  2. Phacoemulsification: Fluidics, Ultrasound Energy, Viscoelastics, and Day One
  3. Biometry, Implant Verification, and Perioperative Safety
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